Product Description Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria When used with acidequilibrated phenol or phenol chloroform, a solution of Guanidine Thiocyanate and betamercaptoethanolAvantor ®, a Fortune 500 company, is a leading global provider of missioncritical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industriesOur portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serveProduct Description Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria When used with acidequilibrated phenol or phenol chloroform, a solution of Guanidine Thiocyanate and betamercaptoethanol
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Guanidine thiocyanate sds sigma-Synonyms Guanidine Isothiocyanate Formula NH 2 C(NH)NH 2 ·HSCN Molecular Weight g/mol CAS# EC# Component Classification Concentration Guanidine Thiocyanate Acute Tox 4;√100以上 guanidine isothiocyanate sds Guanidine thiocyanate sds Acid guanidinium thiocyanatephenolchloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistryIt is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases) This method may take longer than a
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Guanidinium Chloride @ 2550% Acetic Acid @ 1025% NonHazardous Proprietary Ingredients @ Balance Chemical Hazardous Waste Buffer P1 **See note Yale Environmental Health & Safety MANAGEMENT & DISPOSAL OF QIAGEN REAGENTS Reagent Name Chemical Components Management/DisposalIn a highly concentrated guanidine isothiocyanate solution, many proteins unfold and separate into their constituent polypeptides Guanidine Isothiocyanate is widely used to purify nucleic acids from cell extracts Due to its properties as an RNase inhibitor, Guanidine Isothiocyanate Solution is especially useful for the isolation of RNAs from sources rich in RNase, such as pancreas Qiagen AVL Buffer safety data sheet Qiagen RNA extraction kits use AVL buffer AVL buffer has 5070% guanidinium isothiocyanate according to their safety data sheet Their website doesn't let you link directly to the safety data sheet as the url expires after some time;
Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation It is used as a storage buffer for whole blood samples Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies It is used in combination with phenol−chloroform in RNA extractionSDS Strong sequestering and denaturation reagent guanidine thiocyanate is also known as guanidine isothiocyanate, guandinium cyanate, guandine thiocyanate, guanidinium rhodanide, thiocyanic acid with guanidine and guanidine, monothiocyanate , with an acronym of GITC It is the essential reagent for nucleic acids sample preparation inCatalog Number Synonyms Guanidine isothiocyanate Guanidinium thiocyanate;
Thiocyanic acid, compound with Guanidine (11) Recommended Use Laboratory chemicals 2 HAZARDS IDENTIFICATION Target Organs Skin, Respiratory system, Eyes, Gastrointestinal tract (GI), Liver,Purity Limit ≥ 995% (Assay, Ion Chromatography) Grade Biotech Grade Molecular Formula CH 5 N 3 ·HSCN Molecular Weight CAS No MDL No MFCD Appearance White to slightly offwhite crystalline Melting Point 116 121 °C Warnings Irritant!Guanidine Thiocyanate Guanidinium thiocyanate or guanidinium isothiocyanate is used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in molecular biology application of DNA and RNA extraction from cells
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The use of guanidinium isothiocyanate in RNA extraction was first mentioned by Ulrich et al (1977) The method was laborious Therefore, it has been displaced by a singlestep technique, which is known as Guanidinium thiocyanatephenolchloroform extraction, by Chomczynski and Sacchi (1987) 12 , whereby the homogenate is extracted withSafety Data Sheet (SDS) Page 3 of 10 Date of Issue 6 M Guanidine Thiocyanate Buffer Personal precautions, protective equipment and emergency procedures Wear appropriate protective equipment including respiratory protection as conditions warrant Do not touch or walk through spilled material Do not touchAC Synonyms Guanidinium isothiocyanate;
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In the widely used guanidinium thiocyanate phenolchloroform method, the cell extract is extracted with phenol/chloroform at low pH Guanidinium thiocyanate is a chaotropic agent used in protein degradation The principle of this singlestep technique is that RNA is separated from DNA after extraction with acidic solution consisting guanidinium thiocyanate and phenolGuanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate CAS Catalog Number 1290 100 g 1291 500 g 1292 1 kg Storage Store at 28°C Directions for use Guidelines for use (Boom, 1990) Buffers Composition of Washing buffer L2 Dissolve 1 g of Thiocyanate Guanidine (GuSCN) in 100 ml of 01 M Tris hydrochloride, pH 64(M)SDS sections updated 16 Other information For research use only US Federal Regulations This product has been classified according to the hazard criteria of the CPR and the MSDS contains all of the information required by the CPR Component Component CASNo Weight % HAPS data Guanidine isothiocyanate ( 1540 ) Phenol 1052
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Synonym Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, • The denatured protein is suitable for SDSPAGE and western blotting General description Monophasic solution of phenol and guanidine thiocyanate for RNA, DNA, and protein isolation Other Notes For life science research onlyDescription Guanidine thiocyanate is a compound that is widely used for the isolation and extraction of RNA in biochemical research In this capacity, it has been used in many studies relating to cell function, signaling and gene expression Guanidine thiocyanate can also be used to create whole blood lysates for longterm storage or transportation of samplesMaterial Safety Data Sheet Guanidine thiocyanate ACC# Section 1 Chemical Product and Company Identification MSDS NameGuanidine thiocyanate Catalog NumbersAC, AC, , , BP2211, BP SynonymsThiocyanic acid, compd with guanidine (11);
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Product Name Guanidine thiocyanate Cat No AC; Reason for revision SDS sections updated Revision number 11 Revision date 25Apr17 For Research Use Only Not for use in diagnostic procedures References • ECHA http//echaeuropaeu/ SECTION 15 Regulatory information Component US TSCA Phenol 1052 ( 3060 ) Listed Guanidine isothiocyanate ( 1540 ) Listed Ammonium thiocyanate0以上 guanidine isothiocyanate sds Guanidine thiocyanate sds sigma The three salts that can be used are Guanidine thiocyanate, which requires ethanol Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen Ammonium acetate
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Ultrapure enzymegrade guanidine isothiocyanate, guanidine hydrochloride, sodium dodecyl sulfate, restriction endonucleases and X DNA were purchased from BRL (Bethesda, MD) Proteinase K and RNase A were purchased from BoehringerMannheim (Indianapolis, IN) and ARgrade isopropanol was obtained from Mallinckrodt (Paris, KY)Trade Name GUANIDINE THIOCYANATE Signal Word Danger Hazard Statements H302 Harmful if swallowed H314 Causes severe skin burns and eye damage H412 Harmful to aquatic life with long lasting effects Precautionary Statements P260 Do not breathe dust or mist P264 Wash skin thoroughly after handlingGuanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization
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Safety Data Sheet for Guanidinium thiocyanate 0613 Material Safety Data Sheet or SDS for Guanidinium thiocyanate 0613 from Merck for download or viewing in the browser Catalog Number 0613 Product Name Guanidinium thiocyanate Select LanguageMaterial Safety Data Sheet or SDS for Guanidine Thiocyanate CAS Calbiochem 3675 from MilliporeSigma for download or viewing in the browser Catalog Number 3675 Product Name Guanidine Thiocyanate CAS Calbiochem Select LanguageGuanidine Thiocyanate Safety Data Sheet According to Regulation (EC) 1272/08 Issue Date Revision date SECTION 1 Chemical Product and Company Identification 11 Product Identifiers Product Name Guanidine Thiocyanate CAS# EC# RTECS Not available Synonyms Guanidine Isothiocyanate
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Guanidine thiocyanate Revision Date 22May19 Hazard Statements Causes severe skin burns and eye damage Harmful if swallowed, in contact with skin or if inhaled Precautionary Statements Prevention Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this productGuanidinium thiocyanate (GTC) or guanidinium isothiocyanate ( GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in the extraction of DNA and RNA from cells GITC may also be recognized as guanidine thiocyanateConcerning researchers mixing bleach with RNA extraction kits waste These kits contain guanidine salts (eg, guanidine thiocyanate and guanidine hydrochloride) that may produce hazardous gases when combined with bleach (sodium hypochlorite) and/or strong acids Please reference the Bleach Incompatibility Information in Appendix 1 See also the
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UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1) For Research Use Only Chemsrc provides Guanidine thiocyanate(CAS#) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc Articles of Guanidine thiocyanate are included as wellCell lysis buffer for RNA extraction is highly denaturing and is usually composed of phenol and guanidine isothiocyanate RNase inhibitors are usually present in the lysis buffer, since RNases can be very resistant to denaturation and remain active For extraction of DNA the lysis buffer will commonly contain SDS
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Name of substance Guanidine thiocyanate Index No Registration number (REACH) xxxx EC number CAS number Molecular formula C₂H₆N₄S Molar mass 118,2 g/mol SECTION 4 First aid measures 41 Description of first aid measures General notes Take off immediately all contaminated clothingProduct Specifications Guanidine thiocyanate Guanidine Isothiocyanate FormulaCH 5 N 3 HSCN MW g/mol Color/PurityWhite to slightly offwhite crystalline powder Forms aUltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, RNA Extraction This product (s) resides on a Fisher Scientific GSA or
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Guanidinium thiocyanate, Guanidinium isothiocyanate, GTC (eg 25 mM sodium citrate, 0,5 % SDS) Highly chaotropic agent that potently destroys the threedimensional structure of proteins, leading to denaturation Guanidinium (iso)thiocyanate contains potent anionic as well as cationic groups, that form strong hydrogen bonds It, thereforeHowever, using urea or guanidine can determine what procedures you can use to analyze the unfolded protein As an example, you can't use proteins in guanidine to run SDSPAGEManuele Martinelli checkmab srl Dear Pravin try just to dilute a fraction of your eluted protein with the same buffer containg 8M urea 14 (as 10ul eluted 30ul of urea buffer) boil the sample
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OmniPur® Guanidine Isothiocyanate CAS Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents CoA 51OPGuanidine thiocyanate C2H6N4S CID structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicityGuanidine isothiocyanate lysis buffer Next Section 4 M Guanidine isothiocyanate (472g/100ml) 25 mM sodium acetate, pH 60 1 mM EDTA Previous Section « Previous Next Article »
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Guanidine Thiocyanate, Molecular Grade (Guanidinium Thiocyanate) 100g ¥ 7,000 Your price ¥ 7,000 製品概要 製品仕様 関連製品 グアニジンチオシアン酸塩は、高濃度でタンパク質変性剤として機能するため、インタクトなRNA抽出の際のRNaseの阻害剤として最も一般的に使用されGuanidine Thiocyanate is an ultrapure, molecular biology grade reagent It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents It is provided in one bottle containing 500 gH302 H312 H332, H314, H412 ≤100% Section 4 First Aid Measures
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Guanidinium rhodanide, Guanidinium isothiocyanate, Guanidine isothiocyanate, Guanidinium thiocyanate CAS Number Molecular Weight Beilstein Registry Number Linear Formula NH2C (=NH)NH2 HSCNSo I've saved it in (the amazing) internet archive https//web
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